Original DNA template - DNA is a double helix made of two complementary strands. Each strand can be used as a template to create a new DNA molecule. Free DNA nucleotides – needed to form the new ...

thus allowing polymerases access to the DNA template. However, the presence of nucleosomes and the folding of chromatin into 30-nanometer fibers pose barriers to the enzymes that unwind and copy DNA.

Because pol-a/primase always requires CMG function to create the ssDNA template, it could never surpass the DNA helicase (Figure 2b). Without a connecting link, the CMG complex could just "run off ...