Stimulated Emission Depletion (STED) microscopy is a super-resolution imaging technique that overcomes the diffraction limit of conventional optical microscopy. It enables the visualization of ...

A new faster-switching, longer-lasting GFP allows gentler and faster high resolution microscopy on living cells. NIH’s plan to reduce indirect funds faced immense backlash from researchers. A federal ...

STED microscopy uses small fluorescent particles—fluorophores—in the sample that glow (fluorescence) with the help of an excitation laser. A second laser beam with a donut-shaped cross-section ...

In fluorescence microscopy, this ON/OFF separation principle could be implemented to perfection, since molecular fluorescence can be easily switched on and off. In fact, STED and PALM/STORM ...

Researchers enhanced Stimulated Emission Depletion (STED) microscopy by replacing traditional fluorophores with nanographenes, enabling the observation of longer-duration processes, overcoming a ...

Inspired by a super-resolution microscopy technique known as STED, we are showing that it is indeed possible to write features whose dimensions are substantially smaller than the diffraction limit.

The 2014 Nobel Prize in Chemistry was awarded for the development of super-resolved fluorescence microscopy, including STED (Stimulated Emission Depletion) microscopy. This method can be used to ...

Figure 1. Inner membranes of live mitochondria under a STED microscope imaged using the MitoPB Yellow fluorescent marker molecule created by researchers at the Institute of Transformative Bio ...