The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest. It uses the fact that denatured nucleic acids will bind to ...

A Southern blot is a laboratory method used to detect specific DNA molecules from among a many other DNA molecules. The technique was named after its inventor, Edward Southern. As a lab procedure ...

1| Pellet ∼10 8 cells at 300g at 4 °C for 10 min in a centrifuge. The nuclei should be kept cold throughout the procedure. Manipulations and transport to the centrifuge should be done on ice. 2 ...

Foundations | The Birth of the Southern Blot, 1975 Courtesy of Ed Southern Gels used for electrophoresis of nucleic acids and proteins are permeable. This obvious fact didn't dawn on me until I tried ...

View Full Profile. Learn about our Editorial Policies. The main difference between Southern, northern, and western blot techniques is the target molecules. While western blots target proteins in a ...