After IEF in the presence of urea and a nonionic detergent, the IEF gel is equilibrated in sodium dodecyl sulfate (SDS) to prepare the proteins for SDS-PAGE. The method described here 1 uses ...

SDS, 1 μg/ml poly(A), 100 μg/ml salmon sperm DNA, 50% (vol/vol) formamide). This protocol was adapted from “Pulsed-field gel electrophoresis via transverse alternating field electrophoresis ...