HindIII, for example, is an H. influenzae restriction enzyme that recognizes the sequence 5'AAGCTT-3' (upper strand)/3'TTCGAA-5' (lower strand) and cleaves between the two A's on both strands.

DNA restriction enzymes, with their widespread applications in many diagnostic procedures (e.g., RFLP, PCR, etc), are the very cornerstone of recombinant DNA technology. Unfortunately, their ...

3 In 1970, Hamilton Smith and Kent Wilcox from Johns Hopkins University discovered “endonuclease R” (later named HindII), a restriction enzyme isolated from Hemophilus influenza. 1 A year later, ...

Moreover, the specificity of a restriction enzyme is dependent on environmental conditions. For this reason, scientists looked for ways of improving the accuracy of restriction enzymes and of ...

A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

The study, published in the journal Cell Death & Disease, reveals important progress in unraveling how the enzyme ... fetal growth restriction and preeclampsia continue to pose substantial ...