So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort has been spent defining the "correct" conditions that ...

Because housekeeping gene expression can vary considerably among cell types or experimental conditions, our procedure uses multiple ... is used during one-step RT-PCR, we introduced a two-step ...

As the coronavirus that causes the COVID-19 disease spreads across the world, the IAEA, in partnership with the Food and Agriculture Organization of the United Nations (FAO), is offering its support ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...

Real-time PCR detects and quantifies DNA as the reaction progresses. This quantifies the specific amount of DNA present in a sample. The procedure requires specialized thermal cyclers with ...