单细胞测序技术经过了10余年的发展，取得了众多的技术进展与突破。其中，单细胞RNA测序技术中，Smart-Seq方法以其可实现完整mRNA扩增的优势，被广泛的应用。而近期广受关注的另外一个高通量单细胞RNA测序技术由Macosko E团队开发，其研究成果于2015年发表在 ...

The RNA sequencing (RNA-seq) revolution is underway ... is one researcher using RiboFree’s universal rRNA removal method. He recently collaborated on a study investigating the effects of ...

Alternative splicing (AS) of pre-mRNA plays a crucial role in tissue-specific gene regulation, with disease implications due to splicing defects. Predicting and manipulating AS can therefore uncover ...

2017 Cell; Li et al 2020 Current Biology). Recently, we developed a single-nucleus RNA-seq method in flies (McLaughlin, …, Li, 2021 eLife) and applied it to the Fly Cell Atlas (FCA) project, a large ...

This study shows that RNA decay rather than the presence of premature termination codons triggers a TA-like response.

This important study offers a molecular characterization of neurons and glia in the adult nervous system of the fruit fly Drosophila melanogaster. The study focuses on the progeny of a specific set of ...

In this interview, we gain a behind the scenes look at Morgan’s recently published paper in BioTechniques, ‘RNA Extraction and RNA-Sequencing Method for Transcriptomic Analysis of Mycobacterium ...

One technique could image lots of RNA molecules, but only in a thin layer of cells. Another method could image in deep tissue but could only detect three or four molecules in a single sample.

The cells in each dataset are described in UMAP embeddings from RNA-seq, colored by cell types (ncells > 500) and annotated ...