利用 SLAM-seq（thiol (SH)-linked alkylation for metabolic sequencing of RNA，基于硫醇连接的烷基化代谢测序技术）研究新生 mRNA 的成熟。同时，还借助了免疫沉淀（immunoprecipitation）、Western blotting（蛋白质免疫印迹）、IPAfinder 分析内含子多聚腺苷酸化（intronic ...

“RNA-Seq [...] is expected to revolutionize the manner in which eukaryotic transcriptomes are analysed.” Figure 2: Quantifying expression levels: RNA-Seq and microarray compared. Taking all of ...

Transcription-induced RNA mutations include chimeric RNA, alternative splicing, alternative polyadenylation, and RNA editing. The prevalence of RNA mutations may allow the limited number of human ...

The realization that most human genes generate multiple mRNAs encoding divergent protein isoforms via alternative splicing and polyadenylation has revealed extensive regulation that remains to be ...

regulates alternative cleavage and polyadenylation of mRNAs. "Our team has identified a gap in knowledge around the RNA-binding capabilities of C2H2 zinc finger proteins," said Jack Greenblatt ...

A paper published in the journal Cell highlighting a novel genome wide profile of polyadenylation events in human and yeast, performed with Direct RNA Sequencing, demonstrated the importance of ...