We tested this system using 30-ml cultures in shake flasks and were able to scale up the production process using the same transfection procedure irrespective of the final culture volume (5-liter ...

Neurons, neural stem cells, and other slowly dividing cells are notoriously difficult to transfect. With CRISPR-based gene editing gaining momentum, transfection is an essential technique in the ...

Some carrier molecules are designed to help this process along. Metafectene, distributed by Biontex, is a polycationic transfection reagent based on what the company calls “repulsive membrane ...