A restriction map of the 5.3 kb insert is prepared. The recombinant plasmid is digested with the enzymes listed above in single digests, and then several combinations of enzymes are tested in double ...

specific sequences called restriction sites. After a DNA segment has been digested using a restriction enzyme, the resulting fragments can be examined using a laboratory method called gel ...

specific sequences called restriction sites. After a DNA segment has been digested using a restriction enzyme, the resulting fragments can be examined using a laboratory method called gel ...

Restriction sites, like airline hubs, aren't always where you need them, and sometimes they aren't there at all. Recombinase-based methods allow scientists to move pieces of DNA from plasmid to ...

Plasmid DNA (pDNA) has become a cornerstone in the development of advanced therapeutics, including gene therapies, DNA ...