A scaleable method for the liquid-phase separation of plasmid DNA from RNA. In the course of experiments on the potential of nucleic acid compaction for enhancement of chromatographic adsorption ...

The cutting and joining manipulations that underlies gene cloning are carried out by enzymes called restriction endonucleases ... During this week you will carry out restriction digestion of the ...

A technology that uses enzymes to cut and paste together DNA sequences of interest. The recombined DNA sequences can be placed into vectors that carry the DNA into a host cell. In this host cell ...

In step 2, the gene fragment is integrated into a circular piece of DNA, called a plasmid, which is the main delivery vehicle into the cell and contains an antibiotic resistance gene for plasmid ...