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In the last decade, two techniques have revolutionized fluorescence microscopy: the development of genetically encoded fluorophores, such as GFP 5,6 and the invention of two-photon microscopy 7.
The Leica SP8 FALCON (FAst Lifetime CONtrast) is a fast and completely integrated fluorescence lifetime imaging microscopy (FLIM ... spectral hybrid detectors. Photon arrival times are recorded ...
The ability to reactivate nanographenes with inherently high photon numbers makes them ... Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene ...
This means that each electron transition will produce a photon of a different frequency and hence a different colour. This causes line emission spectra to be produced, as shown below. This is not ...