Microtubule/MAPs were purified from rabbit brain extracts by two rounds of temperature-dependent microtubule association and dissociation 47. Supernatants and pellets from each round (20 μg per ...

The rotor should be prewarmed to 37 °C. Remove the supernatant, which contains the microtubule-associated proteins (MAPs); the pellet is the MAP-depleted tubulin polymer. Estimate the tubulin ...