At 45-60°C, the first hot-start activation phase occurs, during which reverse transcriptase is activated and cDNA synthesis is initiated ... In summary, the Invitrogen SuperScript IV UniPrime One-Step ...
Reverse transcription was carried out using SuperScript First-Strand Synthesis System for RT-PCR (Invitrogen) followed by PCR with the Fast Start High Fidelity PCR System (Roche Applied Science ...
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