1 Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA 2 Department of Chemistry, Princeton University, Princeton, NJ 08544, USA Histone post-translational modifications ...

To analyze the success of the experiment, carry out amplification of 1–2 μl of the diluted DNA as a template, using PCR primer pairs that amplify sequences for which the histone modification ...

The basic building blocks of chromatin are nucleosomes, each of which is composed of 146 bp of DNA wrapped around a histone octamer that consists of two copies each of H2A, H2B, H3 and H4.