Conditional gene targeting based on excision or inversion of loxP-flanked DNA segments by Cre recombinase is a powerful technology for the analysis of gene function, but unexpected expression ...

Gene-trapped mouse embryonic stem cell lines are available from several sources. Steps in the production of gene-targeted mice include: Construction of a gene-targeting vector from the isogenic ...

The Transgenic and Knockout Core can construct gene-targeting vectors for the generation of knockout, knockin and conditional knockout mice. A recombineering approach is used, and it typically takes ...

Pluripotent stem cells can be genetically labeled to facilitate differentiation studies. In this paper, we describe a gene-targeting protocol to knock in a GFP cassette into key gene loci in human ...

Gene therapy in the central nervous system ... Here we provide evidence for a novel, noninvasive approach for targeting potential therapeutic factors to the CNS. Genetically-modified hematopoietic ...

However, complicated scenarios put forward new challenge for path planning of multiple UAVs. Therefore, a novel cylindrical coordinate particle swarm optimization (PSO) planner with gene targeting ...

Conditional mutagenesis in mice has recently been made possible through the combination of gene targeting techniques and site-directed mutagenesis, using the bacteriophage P1-derived Cre/loxP ...

You will be redirected to our submission process. The recent development of new gene targeting related tools (e.g. base editors, prime editing, GRAND, pfAGO, SHERLOCK, DETECTR, PAC-MAN, ABACAS, etc.) ...

Scientists at The Hospital for Sick Children (SickKids) have identified a key gene that could lead to next-generation ...

Liu created the particles that were utilized to precisely deliver the gene therapy to cancer cells ... a cancer-specific promoter and a cancer-targeting nanoparticle, we found this therapy ...

RNA pull down, a luciferase reporter assay, biotin-coupled microRNA capture and fluorescence in situ hybridisation were used to evaluate the interaction between CircSERPINE2 and miR-1271-5 p, as well ...