Thus, although four bases are used to synthesize DNA, the nucleotide sequence of any plasmid DNA is in fact composed of six bases as a consequence of this epigenetic phenomenon. The omission of ...

plasmid DNA is often purified on a smaller scale for sequencing and other purposes. With this in mind, we have developed a scaled-down mini-prep protocol based on compaction precipitation.

The community would really benefit from more security awareness. For example, every research sample, such as a tube with a DNA plasmid, has two facets: a computer record that contains information ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

A method for genetically barcoding Drosophila is developed and used to tag defined cell populations in vivo for single-cell transcriptomics experiments and to enable multiplexed behavioral analysis.

The GENE PREP has protocols for DNA purification and extraction from tissues, cells, plants and plasmid preps.

For Smith and other physical scientists, the grail of the government's Human Genome Project (HGP) is not the sequence and analysis of our 3 billion DNA bases, as it is for the biologists, but the ...

The iterative construction process, which involves stitching smaller gene fragments together ... for each construct to identify a sequence-perfect plasmid. In this GEN webinar, Elegen’s Galit ...

Properties such as GC-content, repeats such as poly(A)-tails and loop structures affect sequencing reactions, so any information you can provide will help us select suitable reaction protocol.