By removing a gene to stunt cane toads at their tadpole stage, scientists hope they may have found a way to make the invader ...

4 This technique, known as CRISPR-interference (CRISPRi) allows targeted ‘knockdown’, rather than knockout. “CRISPRi represses the gene of interest, instead of turning it off,” Donnelly ...

Most often, a technique called electroporation is used ... To produce a mouse that is homozygous for the target gene knockout, chimeric mice capable of passing the knockout mutation on to their ...

to knockout and characterize all protein-coding genes in the mouse genome. Knocking out the activity of a gene provides valuable clues about what that gene normally does. However, creating and ...

a technique known as gene knockdown. 8 By delivering dsRNA that are complementary to a target gene’s mRNA, researchers redirect the RNAi pathway from its canonical functions to cleave the target and ...

“Having a complete knockout, you’ll have much stronger phenotypes compared to other techniques,” said Jan Carette, a Stanford University professor who was not involved in the current study, but who ...

Traditional gene knockout methods, including CRISPR-Cas9 ... By fine-tuning gene activity instead of completely disabling ...

The study, by Dr. Nathalie Cartier-Lacave of INSERM in Paris, France and colleagues, was titled, "Hematopoietic Stem Cell Gene Therapy with a Lentiviral Vector in X-Linked Adrenoleukodystrophy." ...