One way to understand the function of a gene is to observe a biological system that lacks that gene. But what is the best system to use? When studying human genes, researchers typically employ ...

1 There are four key steps in the RNAi pathway. When miRNA acts as the guide for RNAi ... researchers redirect the RNAi pathway from its canonical functions to cleave the target and prevent its ...

Both gene knockout and knock-in models are powerful tools widely used in biomedical research to gain important insights into ...

In theory, mutating a gene of interest inside stem cells enables researchers to analyze the effects of that mutation on the development of particular cell types. In the laboratory of Jack Parent at ...

To create a knockout mouse, scientists genetically engineer the animal by disrupting a gene of interest. This may be done by deleting portions of the gene's DNA sequence to make it nonfunctional ...