This approach, called sequence-enabled reassembly ... because now the zinc fingers bind DNA tightly and only a single GFP reassembles. “So if we can get the zinc fingers to have lower affinity ...

1a) to produce in-frame GFP-fragment fusions: Depending on the composition of the amplification buffer, additional Mg 2+ may be required for template DNA above 1 kilobase (kb). Alternatively ...