From synthetic biology and gene therapy to data storage and biofuel production, the demand for synthetic DNA is rising. (1) Chemicals remove the 5’-hydroxyl protecting group. The nucleosides are rich ...

Biology lectures teach students that when a cell’s replication machinery comes together, DNA polymerase takes off down the double-helix like a car on a highway ...

enabling the production of custom DNA sequences of up to 500 nucleotides in length with unprecedented sequence complexity. This breakthrough in enzymatic DNA synthesis overcomes traditional chemical ...

This procedure includes DNA fragmentation (sonication ... using both Roche 454–Compatible and Illumina-Compatible Nextera Enzyme Mixes according to the standard protocol (Fig.

Recently, mechanical fragmentation with or without pharmacologic thrombolysis has been shown to have a role in therapy for patients with massive PE, as well as in those patients who have a ...