4| Transfer 400-μl aliquots of the cell suspension (10 6 –10 7 cells) into as many labeled electroporation cuvettes as needed. Place the loaded cuvettes on ice. 5| Set the parameters on the ...

The facility is fully equipped for microinjections, microsurgery, zygote electroporation, gene targeting and tissue culture, and together with Purdue’s Gene Editing Facility and MilliporeSigma’s ...

However, optimizing electroporation conditions to achieve high-efficiency transfer without affecting cell viability remains a major challenge in cellular therapeutics development. Download this ...

Brief electroporation appears to make T cells more receptive to ... A quick zap of electricity makes T cells more receptive to taking in new genetic material and gene-editing reagents, researchers ...

The Gene Pulser Xcell System is a flexible, modular electroporation system for transfecting every cell type from primary, suspension, and difficult-to-transfect cells, including T cells, to bacteria ...

Gene delivery methods can be mechanical (e.g. microinjection, electroporation or biolistics), chemical (e.g. lipid or nanoparticle carriers) or biological (e.g. viral or bacterial vectors).