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生物通
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CRISPR/Cas 技术打造抗地塞米松的 CAR-T 细胞,为 EBV 相关疾病治疗带来 ...
研究人员用 CRISPR/Cas 技术构建抗 EBV 的 CAR-T 细胞并敲除糖皮质激素受体 ... 如通过 PCR 和流式细胞术检测 CAR 敲入效率,蛋白质免疫印迹法(Western blot)检测 GR 敲除效率,还有多种实验评估细胞的功能,包括细胞毒性、增殖能力和细胞因子分泌等 ...
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