实例说明PolyJet™体外DNA转染试剂对常见细胞系优异的转染效率。 PolyJet ™ 转染试剂和lipofectamine Plus转染试剂对中国仓鼠卵巢(CHO)细胞转染效率的比较。分别使用PolyJet™ (左图)和lipofectamine Plus (右图)将HA标记的β­微管蛋白cDNA转染入中国仓鼠卵巢细胞。异硫氰酸 ...

操作步骤简单：简单易用的操作步骤，可进行反向转染 (Reverse Transfection)，无需复杂优化。 在转染前一天铺细胞，在转染当天 细胞融合度在75%左右。 转染当天，将以无血清培养基稀释 DNA。再加入适量ViaFect™ 转染试 剂与DNA构成适当比例，孵育 5-20min。 加入适量 ...

Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...

In this article, we discuss transformation, bacterial transformation in particular, and how scientists can utilize it to ...

The basic pattern of genomic DNA methylation is established at the time of embryo implantation through a wave of de novo methylation, but CpG islands are protected through a mechanism that ...

First and only immunotherapy to show meaningful overall survival benefit in a Phase 2 trial in patients with advanced ovarian cancer Initiation of trial sites underway for ground-breaking Phase 3 ...

DNA is the molecule that holds the instructions for all living things. DNA achieves this feat of storing, coding and transferring biological information though its unique structure. Replication of ...

Reporter genes are generally joined to a regulatory DNA sequence in an expression vector that is usually propagated in Escherichia coli before transfection into the cell type of interest. 1 A control ...