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DNA analysis is crucial for identifying individuals and their relatives, using technologies like PCR, electrophoresis, and ...
2| Remove 48 μl to serve as the no-template control reaction and add 2 μl of primer pair 1. 3| Add 5.5 μl of genomic DNA at 10 ng/μl to the master mix (Step 1) and mix thoroughly. 4 ...
DNA polymerase (DNAP) is a type of enzyme that is responsible for forming new copies of DNA, in the form of nucleic acid molecules. Nucleic acids are polymers, which are large molecules made up of ...
Then single-stranded binding proteins called gp32 bind to the displaced DNA strand to stabilize it. The recombinase disassembles, allowing a DNA polymerase to bind to the 3’ end of the primer, and DNA ...