One is that the DNA template somehow becomes defective during replication, and causes the polymerase to pause (Figures 3 and 4a ... the checkpoint kinase 1 (Chk1) (Figure 4) (Cimprich & Cortez ...

1 Denaturation: Double-stranded DNA separates into single strands. Annealing ... 6 A combination of highly processive thermostable DNA polymerase and a high-fidelity polymerase with 3′-5′ exonuclease ...

Resulting gaps are filled in by DNA polymerases; this reaction leaves a nick at or flanking the site of repair. An analogous process occurs during chromosomal DNA replication, whereby the 5’-RNA ...

Then single-stranded binding proteins called gp32 bind to the displaced DNA strand to stabilize it. The recombinase disassembles, allowing a DNA polymerase to bind to the 3’ end of the primer, and DNA ...

Researchers used digital polymerase chain reaction assays to measure BRAF V600E or BRAF V600K circulating tumor DNA in ...

These facts underpin the generation and use of DNA fingerprints to identify individuals and their relatives. The DNA is packed inside chromosomes. For example, chromosome no. 3 contains 6.5% of ...

Droplet digital polymerase chain reaction (PCR) measurements of cell-free, circulating tumor DNA (ctDNA) can identify ...

When the mechanisms that replicate or repair mitochondrial DNA (mtDNA) fail, it can cause a wide range of syndromes for which ...