Chromatin immunoprecipitation (ChIP ... to DNA-binding proteins with formaldehyde and mechanically sheared using sonication. The DNA-protein complex is then precipitated with an antibody that ...
Sonicate the resuspended chromatin solution five times for 15 s each at ∼10% power (setting 2.5 on the sonicator, Sanyo Soniprep 150). Place the sample on ice for 1 min between each sonication ...
These modifications increase chromatin accessibility, influencing growth, differentiation, and ion transport, with contrasting effects on normal and colorectal cancer cells. Study: Short-chain ...
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