A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA ... DNA fragments or genes into a plasmid vector, creating a so-called recombinant ...

Thus, this phenomenon is not cell dependent but represents a general characteristic of the TRIP vector. The presence of the central DNA flap in HIV vectors enhances in vivo gene transduction.

First generation vectors may be suitable for applications where transient, high level activity is sufficient, and where immune responses to the vector or transgene are acceptable ... The viral lysate ...