Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

Accumulating evidence from mouse studies suggests that cardiovascular calcification is not simply dependent on serum concentrations of calcium and phosphate, but rather is a regulated process that ...