Design a CRISPR construct to knockout TAT genes in cyanobacteria. Identify the optimal CRISPR plasmid backbone, design and insert the guide RNA (gRNA) into the plasmid, synthesize and insert repair ...

The CRISPR-Cas (Clustered Regularly Interspaced ... electroporation, or plasmid transfection method. 2 Compared to all delivery methods, RNP electroporation exhibited higher on-target editing ...