made it possible to analyse mammalian cells that express autophagy proteins tagged with fluorescent markers. Time-lapse studies provide compelling images which suggest that autophagosome formation ...

LC3-II is used as a marker of autophagy because its lipidation and specific recruitment to autophagosomes provides a shift from diffuse to punctate staining of the protein and increases its ...

The researchers also conducted cellular experiments to investigate autophagy flux using fluorescence-based assays, autophagosome formation markers, and lysosomal activity measurements ...

Recycling takes place in our cells at all times: in a process called autophagy, cell components that are no longer needed are enclosed by membranes and broken down into their basic building blocks.

Get Instant Summarized Text (Gist) Autophagy, particularly through the protein ATG5, plays a crucial role in maintaining brain health by regulating glucose metabolism in neurons. In Purkinje cells ...