Colossal Biosciences said it used ancient DNA from the extinct species to create three dire wolf pups named Romulus, Remus and Khaleesi.

Colossal Biosciences says it used novel gene-editing technology to alter gray wolf DNA to breed the animals. Dire wolves recently featured prominently in the HBO series Game of Thrones.

Colossal, the company looking to bring back the woolly mammoth from extinction has born three dire wolves, an animal extinct for 13,000 years.

The dire wolf, made famous in the hit HBO series "Game of Thrones," has been genetically-engineered by scientists in the U.S. after going extinct around 13,000 years ago.

Dire wolves, made famous by “Game of Thrones,” went extinct some 13,000 years ago. Now, researchers have bred gray-wolf pups that carry genes of their ancient cousins.

Three genetically engineered wolves that may resemble extinct dire wolves are living in an undisclosed secure location in the U.S., according to researchers at Colossal Biosciences. (Video production:

Three white puppies were born in the last six months, each through surrogate domestic dogs who had no idea they were carrying an extinct species.

According to ABC News, the company extracted dire wolf DNA from existing fossils, and then combined it with a close relative. Colossal’s chief scientist Beth Shapiro told the outlet, “We’ve taken a gray wolf genome,

Colossal Biosciences said the first dire wolf pups were born on Oct. 1, 2024, using the CRISPR gene-editing technology and DNA extracted from a 13,000-year-old tooth and a 72,000-year-old ear bone. Domestic dogs served as surrogates for the embryos.